Proteomics Tools

Masses of Common Post-translational Modifications

Modification

Monoisotopic Mass Change

Average Mass Change

Homeserine formed from Met by CNBR treatment

-29.99281

-30.0935

Pyroglutamic acid formed from Gln

-17.02655

-17.0306

Disulphide bond formation

-2.01565

-2.0159

C-terminal amide formed from Gly

-0.98402

-0.9847

Deamidation of Asn and Gln

0.98402

-0.9847

Methylation

14.01565

14.0269

Hydroxylation

15.99491

15.9994

Oxidation of Met

15.99491

15.9994

Formylation

27.99491

28.0104

Acetylation

42.01056

42.0373

Carboxylation of Asp and Glu

43.98983

44.0098

Carboxyamidomethylcysteine (Cam) from Cys (iodoacetamide)

57.02146

57.052

Carboxymethylcysteine (Cme) from Cys (iodoacetic acid)

58.00548

58.0367

Phosphorylation

79.96633

79.9799

Sulphation

79.95682

80.0624

Pyridylethyleysteine (PE-Cys) from Cys (4-vinylpyridine)

105.05785

105.1393

Cysteinylation

119.0041

119.1442

Pentoses (Ara, Rib, Xyl)

132.04226

132.1161

Deoxyhexoses (Fuc, Rha)

146.05791

146.143

Hexosamines (GalN, GlcN)

191.06881

161.1577

Hexoses (Fru, Gal, Glc, Man)

162.05282

162.1424

Lipoic acid (amide bond to lysine)

188.03296

188.3147

N-acetylhexosamines (GalNAc, GlcNAc)

203.07937

203.195

Farnesylation

204.1878

204.3556

Myristoylation

210.19836

210.3598

Biotinylation (amide bond to lysine)

226.0776

226.2994

Pyridoxal phosphate (Schiff Base formed to lysine)

231.02966

231.1449

Palmitoylation

238.22966

238.4136

Stearoylation

266.26096

266.4674

Geranylgeranylation

272.2504

272.4741

N-acetylneuraminic acid (Sialic acid, NeuAc, NANA, SA)

291.09542

291.2579

Gluthathionylation

305.06816

305.3117

N-glycolylneuraminic acid (NeuGc)

307.09033

307.2573

5'-Adenosylation

329.05252

329.2091

4'-Phosphopantetheine

339.07797

339.3294

ADP-ribosylation (from NAD)

541.06111

541.3052

Methyl (Me)

14.01565

14.0269

Formyl (CHO)

27.99491

28.0104

Ehtyl (Et)

28.0313

28.0538

Acetyl (Ac)

42.01056

42.0373

t-Butyl (t-Bu)

56.0626

56.1075

Anisyl

90.04695

90.1246

Benzyl (Bzl)

90.04695

90.1246

Trifluoroacetyl (Tfa)

95.9823

96.0087

N-hydroxysuccinimide (ONSu, Osu)

97.01638

97.0734

T-Butyloxycarbonyl (Boc)

100.05243

100.1173

Benzoyl (Bz)

104.02621

104.1082

4-Methylbenzyl (Meb)

104.0626

104.1515

Thioanisyl

106.02411

106.1912

Benzyloxymethyl (Bom)

120.05751

120.1509

4-Nitrophenyl (Onp)

121.01638

121.0954

Benzyloxycarbonyl (Z)

134.03678

134.1344

2-Nitrobenzoyl (NBz)

149.01129

149.1058

2-Nitrophenylsulphenyl (Nps)

152.98845

153.1614

4-Toluenesulphonyl (Tosyl, Tos)

154.00885

154.1894

Pentafluorophenyl (Pfp)

165.98419

166.0501

Diphenylmethyl (Dpm)

166.07825

166.2224

2-Chlorobenzyloxycarbonyl (Cl-Z)

167.99781

168.5792

2, 4, 5-Trichlorophenyl

177.91438

179.432

2-Bromobenzyloxycarbonyl (Br-Z)

211.94729

213.0305

9-Fluorenylmethyloxycarbonyl (Fmoc)

222.06808

222.2432

Triphenylmethyl (Triyl, Trt)

242.10955

242.3202

2, 2, 5, 7, 8-pentamehylchroman-6-sulphonyl (Pmc)

266.09767

266.3611


Mass Spectrometry in Biology and Medicine by A.L. Burlingame, Steven A. Carr, Michael A. Baldwin.
ISBN 0-8960-3799-1. Humana Press (1999).

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