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Select a Topic Below:
- Protein Sample Preparation
- Protein Gel Separation and Recovery
- Mass Spec Standards

Protein Sample Preparation:
Tips for overcoming problems with MALDI protein identification
           So, you have gone through days of grueling lab work and tomes of protocols to prepare a sample for the all telling MALDI analysis. When the...
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author: | date created: Jun 01, 2012 | comments: 0
Quantifying protein in samples containing SDS
Question from the forums:
Hi everybody,
I...
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author: | date created: May 23, 2011 | comments: 0
Unique uses for albumin and IgG depletion
Many researchers today are interested in the proteomes of tissue samples from many sources. One problem that is faced when analyzing samples derived from various types of sera is the presence of album...
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author: | date created: May 06, 2011 | comments: 0
Protein Gel Separation and Recovery:
Do you have precast gels?

Yes.  We offer precast mini gels that fit the Bio-Rad and Invitrogen mini gel systems.  The gels are currently offered in 7.5%, 10%, 12%, and 15%.  Comb options included are 10 we...
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author: | date created: Mar 17, 2011 | comments: 0

Why does my silver stain take an extremely long time to develop

There is probably too much water being used in between reagents A, B, and C.  Less than 10 mL of water should be used and wash times should be less than 2-3 seconds.  There is also the...
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author: | date created: Mar 17, 2011 | comments: 0

How critical are the incubation times when using the Silver Stain Kit?

In order to maintain gel-to-gel reproducibility, incubation times and rinse times should be carefully controlled.


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author: | date created: Mar 17, 2011 | comments: 0
Mass Spec Standards:
Do your standards require additional clean up prior to mass spectrometry?

No, our standards are HPLC-purified and are fully mass spec compatible.


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author: | date created: Mar 17, 2011 | comments: 0
How pure are your standards?

Our standards are > 95% pure.


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author: | date created: Mar 17, 2011 | comments: 0
Are your standards quantitative?

No, our standards are NOT intended to be quantitative.


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author: | date created: Mar 17, 2011 | comments: 0
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