Silver Stain Kit

overview | features and specs | data sets | literature

Product Overview
Silver staining of proteins is a useful technique for the detection of minor protein components that are difficult to visualize by standard Coomassie blue stains. Silver stains are 50 to 100-fold more sensitive than Coomassie and thus capable of detecting nanogram quantities (~5 ng) of protein on gels in comparison to Coomassie stains (20 - 40 ng).

The Protea Silver Stain formulation is unique in that the staining process can be completed in about 35 minutes (including the 15 min. fixing step) without sacrificing the sensitivity of detection. In addition, the silver stain kit is compatible with MALDI analysis.
Features and Specifications
Features:
  • 35 minutes from fixation to visualization of protein bands
  • Stable at room temperature for up to 6 months
  • Sensitivity of ~5 ng
  • Mass Spec tested and fully compatible (after silver destaining step)
  • Certified Mass Spec Grade

Research Applications:
  • Biomarker discovery
  • Protein identification and characterization
  • Proteomics method development

Specifications:
  • This product is for R&D use only; it is not for drug, household, or other uses.

Data Sets
62.5 ug of E. Coli lysate was separated using isoelectric focusing on a pH 4-7 IPG strip. The sample was then separated by SDS-PAGE on a 10% ProteaGel (PG-402D) and stained using Protea's Silver Stain Kit (SS-100).


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Literature
***Please to download documentation.

Silver Stain Kit Availability
This product is discontinued and no longer for sale.

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