ProteaPrep Protease Inhibitor Cocktail

overview | features and specs | data sets | literature

Product Overview
The ProteaPrep Protease Inhibitor Cocktail is an easy-to-use reagent for the inhibition or protease activity during biological sample preparation to prevent degradation of sample proteins. We recommend that the ProteaPrep Protease Inhibitor Cocktail be used with our ProteaPrep Bacterial and Mammalian Cell Lysis Kit (SP-800), ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade (SP-810), and the ProteaPrep Zwitterionic Cell Lysis Kit, Mass Spec Grade (SP-816) for the rapid and efficient recovery or proteins from cell cultures, lines, and tissue specimens. The Protease Inhibitor Cocktail is provided as a lyophilized powder for a long shelf life and simple, rapid reconstitution and usage.
Features and Specifications
  • Ready-to-use lyophilized powder permits quick and easy preparation of protease inhibitor cocktail mix.
  • Contains inhibitors of aminopeptidases and serine-, cysteine-, metallo-, and aspartic acid- proteases.

Research Applications:
  • Inhibition of protein degradation during protein extraction and purification from cell lines, cultures, or tissues
  • Purification of biologically active proteins and enzymes
  • Extraction of proteins for 2-D gels, immunoblot and ELISA analysis

ProteaPrep Protease Inhibitor Cocktail contains the following mixture of inhibitors and their specificity towards various proteolytic activities as described below.

AEBSF - 20mM
(4-(2-Aminoethyl) benzenesulfonyl fluoride)
Protease Class
inhibits serine proteases such as trypsin and chymotrypsin.

EDTA - 10mM
(Ethylenediaminetetraacetic acid)
Protease Class
inhibits metalloproteases.

Bestatin - 1.3mM
Protease Class
inhibits aminopeptidases, such as leucine and alanyl aminopeptidases.

E-64 - 140µM
(N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide
Protease Class
inhibits cysteine proteases, such as calpain, papain, cathepsin B, and cathepsin L.

Leupeptin - 10µM
Protease Class
inhibits serine and cysteine proteases, such as plasmin, trypsinogen, urokinase, and kallikrein.

Aprotinin - 3µM
Protease Class
inhibits trypsin and human leukocyte elastase.

*Concentrations are based on a 10X Protease Inhibitor Solution*
Data Sets

(click image(s) above to enlarge)
***Please to download documentation.

ProteaPrep Protease Inhibitor Cocktail Availability
This product is discontinued and no longer for sale.

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