ProteaPrep Non-ionic Cell Lysis Kit, Mass Spec Grade

overview | features and specs | data sets | literature

Product Overview
The ProteaPrep Non-ionic Cell Lysis Kit, Mass Spec Grade has been specially designed for the efficient recovery of purified protein lysates from biological samples. The ProteaPrep Lysis Buffer is a proprietary formulation containing a mixture of salts and non-denaturing detergents that has been optimized for efficient solubilization, extraction, and recovery of most proteins during cell lysis. Because the ProteaPrep Non-ionic Cell Lysis Kit is free of lysozyme and harsh detergents (e.g. SDS), the simple protocol permits recovery of a cell lysate that is free from artifactual protein contaminants and that contains soluble, native proteins for downstream characterization and biological testing.

The ProteaPrep Non-ionic Cell Lysis Kit features the Progenta Non-ionic Acid Labile Surfactant I (NALS I), a powerful acid cleavable detergent that alleviates the problems commonly associated with SDS and other detergents in proteomics studies. After completing the cell lysis, the cell extract solution will not interfere with downstream sample preparation and analysis by techniques such as mass spectrometry, enzymatic digestions, 2D gel electrophoresis, and protein quantitation (e.g. Bradford and Lowry assays).
Features and Specifications
Features:
  • Cell lysate is free of lysozyme and harsh, denaturing detergents (e.g. SDS)
  • Compatible with mass spectrometry, enzymatic digestions and 2D gel electrophoresis

Research Applications:
  • Protein extraction and purification from cell lines, cultures, and tissues
  • Purification of biologically active proteins and enzymes
  • Shotgun proteomics analysis of complex protein samples
  • Analysis of biological samples by 2D gel electrophoresis
  • Extraction of proteins for Immunoblot and ELISA analysis

Specifications:
  • ProteaPrep Non-ionic Cell Lysis Reagent – Lyophilized material until reconstituted then becomes a colorless liquid
  • Progenta Non-ionic Acid Labile Surfactant I (NALS I): critical micellular concentration (CMC) = 2.1 mM
  • This product is for RandD use only; it is not for drug, household, or other uses.

Data Sets

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Literature
***Please to download documentation.

ProteaPrep Non-ionic Cell Lysis Kit, Mass Spec Grade Availability
ProteaPrep Non-ionic Cell Lysis Kit, Mass Spec Grade
ProteaPrep Non-ionic Cell Lysis Reagent (SP-814-10mL) and 10X TBS (SP-840-10mL)
***this product must be shipped cold, requiring 1 or 2 day shipping
SP-817-10kit10kit$176.00add to cart


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ProteaPrep Non-ionic Cell Lysis Kit, Mass Spec Grade

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