ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade

overview | features and specs | data sets | literature

Product Overview
The ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade has been specially designed for the efficient recovery of purified protein lysates from biological samples. The ProteaPrep Anionic Cell Lysis Kit is a proprietary formulation containing a mixture of salts, glycerol, and an acid labile surfactant that has been optimized for efficient solubilization, extraction, and recovery of proteins during cell lysis. Because the ProteaPrep Anionic Cell Lysis Kit is free of lysozyme and harsh detergents (e.g. SDS), the product permits recovery of a cell lysate that is free from artifactual protein contaminants and promotes efficient sample protein solubilization.

The ProteaPrep Anionic Cell Lysis Kit features the Progenta Anionic Acid Labile Surfactant II (AALS II), a powerful acid cleavable detergent that alleviates the problems commonly associated with SDS and other detergents in proteomics studies. After completing the cell lysis, the cell extract solution can be adjusted to a pH of 2.5 - 3 (by TFA, formic acid, etc.) and incubated for 10-20 minutes to fully cleave the AALS into small organic molecules that do not exhibit surfactant activity or interfere with downstream sample preparation and analysis by techniques such as mass spectrometry, enzymatic digestions, 2D gel electrophoresis, and protein quantitation (e.g. Bradford and Lowry assays).
Features and Specifications
Features:
  • Cell lysate is free of lysozyme and harsh denaturing detergents (e.g. SDS)
  • Proprietary anionic acid labile surfactant promotes solubilization of sample proteins with the benefit of rapid detergent degradation under acidic conditions
  • Compatible with mass spectrometry, enzymatic digestions and 2D gel electrophoresis

Research Applications:
  • Protein extraction and purification from cell lines, cultures, and tissues
  • Purification of biologically active proteins and enzymes
  • Shotgun proteomics analysis of complex protein samples
  • Analysis of biological samples by 2D gel electrophoresis
  • Extraction of proteins for immunoblot and ELISA analysis

Specifications:
  • Cell Wash Buffer - Colorless liquid

    ProteaPrep AALS Cell Lysis Reagent A - Colorless liquid

    ProteaPrep AALS Cell Lysis Reagent B - White film
  • Progenta Anionic Acid Labile Surfactant II (AALS II): critical micellular concentration (CMC) = 1.9 mM
  • This product is for R&D use only; it is not for drug, household, or other uses.

Data Sets

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Literature
***Please to download documentation.

ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade Availability
ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade
ProteaPrep Cell Lysis Kit MSG Reagent A (SP-811-10mL), ProteaPrep Cell Lysis Kit MSG Reagent B (SP-812-10mL), and 10X TBS (SP-840-10mL)
***this product must be shipped cold, requiring 1 or 2 day shipping
SP-810-10kit10kit$176.00add to cart
ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade
ProteaPrep Cell Lysis Kit MSG Reagent A (SP-811-25mL), ProteaPrep Cell Lysis Kit MSG Reagent B (SP-812-25mL), and 10X TBS (SP-840-25mL)
***this product must be shipped cold, requiring 1 or 2 day shipping
SP-810-25kit25kit$374.00add to cart


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ProteaPrep Anionic Cell Lysis Kit, Mass Spec Grade

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