ProteaPrep Albumin and IgG Depletion Sample Prep Kit

overview | features and specs | data sets | literature

Product Overview
A major problem in the analysis of the human serum proteome is the inhibition of the detection of lower abundance proteins by higher abundance proteins, such as albumin (HSA) and immunoglobulin G (IgG). Together these proteins comprise ~75% of the total serum protein content. The selective removal of these two major serum proteins permits for a more effective analysis of the proteome.

Affinity-based depletion strategies are often employed to selectively remove high abundance proteins, such as HSA and IgG, to concentrate the lower abundance proteins and facilitate their analysis. Traditionally, antibody-based schemes are used to produce affinity-binding ligands for HSA and IgG, where monoclonal or polyclonal antibodies are produced in a source animal, purified, and then bound to a surface (e.g. bead or well) for preparation of serum samples. Variability in the production of these antibodies leads to a wide variety of specificities and selectivities, as the binding of the ligand to the HSA or IgG is dependent upon the availability of the epitope, the binding affinity of the antibody, and the homogeneity of the antibody population. Thus, the efficiencies for antibody-based depletion strategies for the selective removal of high abundance proteins in human serum can vary broadly, from 70 to 95%, and can dramatically affect the reproducibility of studies of the human serum proteome.

The ProteaPrep Albumin and IgG Depletion Sample Prep Kit (SP-240) features a revolutionary new approach to affinity-based serum albumin and IgG removal that utilizes a non-antibody-based method. The ProteaPrep Albumin and IgG Depletion capture ligands are recombinant proteins that exhibit excellent batch-to-batch reproducibility and are highly purified for robust, efficient removal of albumin and IgG from human serum samples. The net result is that the functionalized beads irreversibly bind and remove the albumin and IgG from serum in a highly efficient and rapid manner. Depletion efficiencies are >99% for the selective removal of both albumin and IgG in a single SpinTube from serum samples in less than 20 minutes.
Features and Specifications
  • >99% efficiency for depletion of both human serum albumin and IgG
  • Simple and easy to use - process samples in less than 20 minutes
  • New recombinant affinity ligand permits highly specific and reproducible albumin and IgG removal to facilitate analysis of lower abundance serum proteins
  • Disposable, cost-effective SpinTube design eliminates the risk of carryover or sample contamination
  • Effectively remove albumin and IgG in one step

Research Applications:
  • Human serum proteome analysis
  • Depletion of albumin and IgG from serum and plasma samples
  • Preparation of serum samples for analysis by SDS-PAGE, 2D gel electrophoresis, and mass spectrometry

Proprietary recombinant affinity ligand for human albumin and IgG is covalently bound to 10µm spherical silica beads with 300Å pores.

This product is for RandD use only, not for drug, household, or other uses.
Data Sets

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***Please to download documentation.

ProteaPrep Albumin and IgG Depletion Sample Prep Kit Availability
This product is discontinued and no longer for sale.

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