ProteaPrep IgG Depletion Sample Prep Kit

overview | features and specs | data sets | literature

Product Overview
Immunoglobulins comprise approximately 15 - 20% of the blood serum protein, with immunoglobulin G (IgG) constituting up to 75% of the serum immunoglobulins in humans. IgG antibodies are tetramers with an average molecular weight of 150 kDa, and they are comprised of two identical heavy chains (~50 kDa each) and two identical light chains (~25 kDa each). The two heavy chains are linked to each other and to a light chain each by disulfide bonds, and the resulting tetramer has two identical halves which together form the Y-like characteristic shape of the antibody.

Affinity-based depletion strategies are often employed to selectively remove high abundance proteins, such as HSA and IgG, to concentrate the lower abundance proteins and facilitate their analysis. Traditionally, antibody-based schemes are used to produce affinity-binding ligands for HSA and IgG, where monoclonal or polyclonal antibodies are produced in a source animal, purified, and then bound to a surface (e.g. bead or well) for preparation of serum samples. Variability in the production of these antibodies leads to a wide variety of specificities and selectivities, as the binding of the ligand to the HSA or IgG is dependent upon the availability of the epitope, the binding affinity of the antibody, and the homogeneity of the antibody population. Thus, the efficiencies for antibody-based depletion strategies for the selective removal of high abundance proteins in human serum can vary broadly, from 70 to 95%, and can dramatically affect the reproducibility of studies of the human serum proteome.

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The ProteaPrep IgG Depletion Sample Prep Kit (SP-220) features a revolutionary new approach to affinity-based serum immunoglobulin removal that utilizes a non-antibody-based method. The ProteaPrep IgG Depletion capture ligand is a recombinant protein that binds with high affinity to IgG, exhibits excellent batch-to-batch reproducibility, and is highly purified for robust, efficient removal of IgG from human serum samples. The net result is that the functionalized beads irreversibly bind and remove the IgG from serum in a highly efficient and rapid manner. Depletion efficiencies are >99% for the selective removal of IgG from serum samples in less than 20 minutes.

Features and Specifications
  • >99% efficiency for depletion of human IgG
  • Simple and easy to use - process samples in less than 20 minutes
  • New recombinant affinity ligand permits highly specific and reproducible IgG removal to facilitate analysis of lower abundance serum proteins
  • Disposable, cost-effective SpinTube design eliminates the risk of carryover or sample contamination

Research Applications:
  • Human serum proteome analysis
  • Depletion of IgG from serum and plasma samples
  • Preparation of serum samples for analysis by SDS-PAGE, 2D gel electrophoresis, and mass spectrometry

  • Proprietary recombinant affinity ligand for human IgG is covalently bound to 10µm spherical silica beads with 300Å pores.
  • This product is for RandD use only, not for drug, household, or other uses.

Data Sets

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***Please to download documentation.

ProteaPrep IgG Depletion Sample Prep Kit Availability
ProteaPrep IgG Depletion Sample Prep Kit
24 SpinTubes with functionalized beads, 25 mL Bead Activation Buffer (SP-290-25mL)
***this product must be shipped cold, requiring 1 or 2 day shipping
SP-220-24kit24kit$835.65add to cart

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ProteaPrep IgG Depletion Sample Prep Kit

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