TiO2 SpinTips Sample Prep Kit

overview | features and specs | data sets | literature

Product Overview
The Titanium Dioxide (TiO2) SpinTip Sample Prep Kit contains tips and reagents for enrichment of phosphopeptides from protein digest samples. The newly formulated reagent solutions supplied with Protea's TiO2 Sample Prep Kits are specially formulated to maximize the retention of phosphorylated peptides, while simultaneously minimizing the retention of non-phosphorylated peptides.

The SP-154-24 kit contains TiO2 SpinTips for 20-1000 µL volumes, 25 mL TiO2 Reconstitution and Wash 1 solution, 25 mL of Wash 2 solution, 25 mL of TiO2 Elution solution, and 8 SpinTip Adaptors.

The SP-154-96 kit contains TiO2 SpinTips for 20-1000 µL volumes, 50 mL TiO2 Reconstitution and Wash 1 solution, 50 mL of Wash 2 solution, 50 mL of TiO2 Elution solution, and 8 SpinTip Adaptors.

Application Note:
Phosphoproteomics of HeLa cells
Features and Specifications
Features:
  • Binding capacity: 1000µg/tip (50-1000µL sample solution).
  • Centrifugal sample preparation format for fast and simple sample prep.

Research Applications:
  • Enrichment of phosphopeptides from complex peptide samples.
  • High throughput sample preparation applications for proteomics analyses.

Specifications:
  • Binding capacity: 1000µg
  • Amount of TiO2 matrix in a tip: 37mg
  • Sample volume: 50-1000µL

Data Sets
The re-formulated TiO2 SpinTip Sample Prep Kit was compared to the previous TiO2 SpinTip Sample Prep Kit and C18 SpinTips Sample Prep Kit (# SP-150). The SpinTips were tested with a complex protein digest mixture spiked with 100 pmol of the following phosphopeptide standards (# PS-180): angiotensin II (1126 Da), cholecystokinin (1331 Da), and calcitonin (1801 Da). The complex protein mixture which consisted of the following tryptically digested proteins: bovine serum albumin (BSA), ovalbumin, and transferrin. The samples were processed with the TiO2 and C18 SpinTips and subsequently spotted directly onto a MALDI target followed by CHCA matrix (# CMS-100).


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Literature
***Please to download documentation.

TiO2 SpinTips Sample Prep Kit Availability
This product is discontinued and no longer for sale.

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