C4 SpinTips Sample Prep Kit

overview | features and specs | data sets | literature

Product Overview
The C4 SpinTips Sample Prep Kit contains tips and reagents for reversed phase solid phase extraction of medium and large protein samples for electrospray or MALDI mass spectrometry. The kit is effective in concentrating and desalting small and large proteins resulting in mass spectra with better S/N and reduced salt adducts.

The SP-152-24 kit contains 24 C4 SpinTips for 20-1000µL volumes, 25mL RP Reconstitution and Rinse Solution, 25mL RP Equilibration Solution, 25mL RP Elution Solution, and 8 SpinTip Adaptors.

The SP-152-96 kit contains 96 C4 SpinTips for 20-1000µL volumes, 50mL RP Reconstitution and Rinse Solution, 50mL RP Equilibration Solution, 50mL RP Elution Solution, and 8 SpinTip Adaptors.
Features and Specifications
Features:
  • High throughput sample preparation.

Research Applications:
  • Effective desalting and detergent removal from medium and large protein samples.
  • Desalting proteins from cell lysates.
  • Concentration of proteins extracted from dilute solutions.
  • Clean-up of proteins electroeluted from polyacrylamide gels.
  • Fractionation of protein mixtures.

Specifications:
  • Maximum Binding capacity: 800 µg
  • Minimum Binding capacity: 250 ng
  • Amount of C4 beads in a tip: 10 mg
  • Sample volume: 20-1000 µL

Data Sets
A sample of bovine serum albumin (BSA) was denatured and processed with a C4 SpinTip according to Protocol 1. A total of 500 pmol of the sample was processed and recovered in 50 µL of elution solution, and 1 µL of this solution was directly spotted on a MALDI plate pre-spotted with CHCA matrix (cat# CMS-100).


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Literature
***Please to download documentation.

C4 SpinTips Sample Prep Kit Availability
C4 SpinTips Sample Prep Kit
C4 SpinTips (SP-152-96), two RP Reconstitution and Rinse Solutions (SP-900-25mL), two RP Equilibration Solutions (SP-901-25mL), two RP Elution Solutions (SP-902-25mL), and 8 SpinTip Adaptors (SP-990-8)
SP-152-96kit96kit$427.30add to cart


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C4 SpinTips Sample Prep Kit

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