C18 ProteaTip Sample Prep Kit, 10-200 µL

overview | features and specs | data sets | literature

Product Overview
The C18 ProteaTips Sample Prep Kit contains tips and reagents for desalting of peptide samples for MALDI- and ESI-MS. The kit is effective in removing salts from protein digests resulting in mass spectra with improved S/N and reduced adducts. The C18 beads are immobilized on the inner surface of a pipette tip providing a large surface area for sample capture.

The SP-129-24 kit contains C18 ProteaTips for 10-200µL volumes, 25 mL RP Reconstitution and Rinse Solution, 25 mL RP Equilibration Solution, and 25 mL RP Elution Solution.

The SP-129-96 kit contains C18 ProteaTips for 10-200µL volumes, 50 mL RP Reconstitution and Rinse Solution, 50 mL RP Equilibration Solution, and 50 mL RP Elution Solution.
Features and Specifications
Features:
  • Effective in removal of salts that reduce S/N of peptide mass spectra
  • Low backpressure during sample processing
  • Binding Capacity: 2-4µg

Research Applications:
  • Effective desalting and detergent removal from biomolecule samples
  • Clean-up of peptides from in-gel digested proteins
  • Fractionation of complex peptide samples

Specifications:
  • 10-200µL ProteaTips:
  • Binding capacity: 2-4µg
  • Amount of C18 beads in a tip: 75µg
  • Sample volume: 10-200µL

Data Sets
C18 ProteaTip clean-up of an in-gel protein digest:

The dual benefits of improved signal-to-noise (S/N) and increased sensitivity (144 vs. 1370 counts) synergistically assist in increasing the number of discernible peptide peaks in the MS spectrum in B.


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Literature
***Please to download documentation.

C18 ProteaTip Sample Prep Kit, 10-200 µL Availability
This product is discontinued and no longer for sale.

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