TiO2 ProteaTip Sample Prep Kit, 1-10 µL

overview | features and specs | data sets | literature

Product Overview
The Titanium Dioxide (TiO2) ProteaTip Sample Prep Kit, 1-10 µL contains tips and reagents for enrichment of phosphopeptides from protein digest samples. The reagent solutions supplied with Protea’s TiO2 Sample Prep Kits are specially formulated to maximize the retention of phosphorylated peptides, while simultaneously minimizing the retention of non-phosphorylated peptides.

The SP-124-24 kit contains 24 TiO2 ProteaTips for 1-10 µL volumes, 10 mL TiO2 Reconstitution and Wash 1 Solution, 10 mL of TiO2 Wash 2 Solution, and 10 mL of TiO2 Elution Solution.

The SP-124-96 kit contains 96 TiO2 ProteaTips for 1-10 µL volumes, 25 mL TiO2 Reconstitution and Wash 1 Solution, 25 mL of TiO2 Wash 2 solution, and 25 mL of TiO2 Elution solution.

Features and Specifications
Features:
  • Enrichment of phosphopeptides in protein digest samples.
  • Low backpressure during sample processing.
  • Binding capacity: 1-2µg.
  • Amount of TiO2 beads in a tip: 30µg.

Research Applications:
  • Enrichment of phosphopeptides from in-gel digested proteins for identification of protein phosphorylation sites by mass spectrometry.
  • Enrichment of phosphopeptides from complex protein/peptide samples.

Specifications:
  • Binding capacity: 1-2µg
  • Amount of TiO2 beads in a tip: 30µg
  • Sample volume: 1-10µL

Data Sets
Enrichment of phosphopeptides using Titanium Dioxide ProteaTips:

A sample of 100µg bovine β-casein was enzymatically digested in-solution overnight with trypsin using an ammonium bicarbonate buffer system. An aliquot containing 1.0µg of digest was processed using a C18 ProteaTip and a 0.2µg aliquot processed using a TiO2 ProteaTip. The resulting MS spectra obtained are shown in Figure 1 below. A total of 30 MS/MS spectra of the most intense peaks in the MS spectra from both tips were collected and phosphopeptides identified from these.


(click image(s) above to enlarge)
Literature
***Please to download documentation.

TiO2 ProteaTip Sample Prep Kit, 1-10 µL Availability
This product is discontinued and no longer for sale.

...innovation for bioanalytics