ZIC-HILIC® ProteaTip Sample Prep Kit, 1-10 µL

overview | features and specs | data sets | literature

Product Overview
The ZIC-HILIC® (Zwitterionic Hydrophilic Interaction Liquid Chromatography) ProteaTip Sample Prep Kit contains tips and reagents for enrichment of glycosylated and hydrophilic peptides from protein digest samples. The proprietary reagents supplied with the ZIC-HILIC® ProteaTip Sample Prep Kit are specifically formulated to maximize the retention of glycosylated and hydrophilic peptides while simultaneously minimizing the retention of non-glycosylated and hydrophobic peptides. The kit is also effective in removing salts and detergents from protein digests resulting in MALDI-MS spectra with better S/N, and less salt adducts in the mass spectra.

The SP-120-24 kit contains 24 ZIC-HILIC® ProteaTips for 1-10 µL volumes, 10 mL ZIC-HILIC® Reconstitution Solution, 10 mL ZIC-HILIC® Equilibration and Rinse Solution, and 10 mL ZIC-HILIC® Elution Solution.

The SP-120-96 kit contains 96 ZIC-HILIC® ProteaTips for 1-10 μL volumes, 25 mL ZIC-HILIC® Reconstitution Solution, 25 mL ZIC-HILIC® Equilibration & Rinse Solution, and 25 mL ZIC-HILIC® Elution Solution.


Features and Specifications
Features:
  • Effective desalting and detergent removal from peptide samples.
  • Clean-up of peptides from in-gel digested proteins.
  • Enriches glycosylated peptides.
  • Low backpressure during sample processing.
  • The ZIC-HILIC® beads (manufactured by SeQuant) are porous silica based and have permanent zwitterionic groups attached.
  • Analyte capture on the ZIC-HILIC beads is based on the hydrophilic interactions between the analyte and the hydrophilic zwitterionic stationary phase.

Research Applications:
  • Enrichment of hydrophilic and glycosylated peptides.
  • Effective desalting and detergent removal from peptide samples.
  • Clean-up of peptides from in-gel digested proteins.

Specifications:
  • Binding capacity: 1-2µg.
  • Amount of ZIC-HILIC beads in a tip: 30µg.
  • Sample volume: 1-10µL.

Data Sets

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Literature
***Please to download documentation.

ZIC-HILIC® ProteaTip Sample Prep Kit, 1-10 µL Availability
This product is discontinued and no longer for sale.

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