ProteaGel™ Stain Kit

overview | features and specs | data sets | literature

Product Overview
Polyacrylamide gel matrix is formed by the polymerization of acrylamide and the cross-linker bis-acrylamide. Polyacrylamide gel matrixes act as a molecular sieve for the electrophoretic separation of proteins. To visualize the separated proteins, the gel matrix may be treated with several gel staining options.

The ProteaGel Stain Kit provides SuperBlue Coomassie R250, ProteaGel Silver Stain, and ZincPlus Stain Kit as staining options. Protea's SuperBlue R250 stain is a traditional Coomassie stain, which is provided as a 10X concentrate solution. Silver staining of proteins is a useful technique for the detection of minor protein components that are difficult to visualize by the traditional Coomassie blue stains. Silver stain is 50-100 fold more sensitive than Coomassie stains and has the capability of detecting nanogram quantities of protein. Unlike Coomassie and silver stains, the ZincPlus Stain is a negative stain, which produces a whitish opaque background in the gel, while the protein bands themselves remain unstained. The inherent benefit of a negative stain is that the protein band does not contain any dye or stain to present as a contaminant for downstream sample processing and analysis.
Features and Specifications
Features:
  • Wide detection range available
  • ZincPlus Stain is a negative stain
  • Silver and ZincPlus stains are reversible

Research Applications:
  • Visualization of proteins after one- or two-dimensional gel electrophoresis
  • Visualization of proteins separated on IPG strips
  • Protein Quantitation of gel separated proteins by densitometry

Specifications:
  • Colorless liquid solution (SS-100-kit and ZS-100-kit)
  • Blue liquid solution (SB-R250-250mL)
  • This product is for RandD use only; it is not for drug, household, or other uses.

Data Sets

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Literature
***Please to download documentation.

ProteaGel™ Stain Kit Availability
This product is discontinued and no longer for sale.

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