Endoproteinase Glu-C Digestion Kit

overview | features and specs | data sets | literature

Product Overview
Endoproteinase Glu-C, also known an V8, is a serine endoproteinase that is isolated from Staphylococcus aureus V8. Endoproteinase Glu-C is highly purified, resulting in a product suitable for proteomics work.

The specificity of Glu-C is primarily determined by the buffer pH and composition. In ammonium bicarbonate buffer (pH 7.8), Glu-C preferentially cleaves glutamyl bonds. In phosphate buffers (pH 7.8), Glu-C will cleave at both glutamyl and aspartyl bonds.

However, the presence of a proline residue on the carboxy side of the bond inhibits cleavage. Glu-C is used in proteomics for peptide mapping and protein sequence work due to its highly specific cleavage of peptides resulting in a limited number of fragments.
Features and Specifications
Features:
  • Certified mass Spec Grade enzyme for proteomics applications
  • Quality Assurance tested for specificity and performance
  • Contains all of the necessary reagents for reduction, alkylation, and digestion of protein samples

Research Applications:
  • Protein identification and characterization by LC-MS/MS
  • De novo sequencing of unique proteins
  • Peptide mass fingerprinting by MALDI-MS


Specifications:
  • Lyophilized powder (contains enzyme stabilizers)
  • This product is for R&D use only, not for drug, household, or other uses.

Data Sets
GLU-C Protease is manufactured using highest quality reagents and tested for protease activity before and after lyophilization with ACTH (1-14) peptide as a substrate. Additionally, samples were verified for MS compatibility.


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Literature
***Please to download documentation.

Endoproteinase Glu-C Digestion Kit Availability
Endoproteinase Glu-C Digestion Kit
Glu-C, Certified MS Grade (PE-102-5), Digestion Buffer (FC-499-1), Dithiothreitol (FC-490-1), and two Iodoacetamides (FC-491-1)
***this product must be shipped cold, requiring overnight shipping
PE-152-5kit5kit$149.34add to cart


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Endoproteinase Glu-C Digestion Kit

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