GPR Electroelution Buffer IV, Tris-Glycine Plus

overview | features and specs | data sets | literature

Product Overview
The Gel Protein Recovery (GPR) Electroelution Buffer IV is specially formulated to optimize the extraction of intact hydrophobic proteins from polyacrylamide gel plugs by electroelution. The GPR Electroelution Buffer IV features Protea's Anionic Acid Labile Surfactant II (AALS II) to provide a safe alternative to SDS-based electroelution buffers with the added benefit of increased compatibility with mass spectrometry. The AALS II detergent is quickly and efficiently degraded under acidic conditions into chemical species that do not associate with the sample or possess surfactant properties. We recommend that the GPR Electroelution Buffer IV be used with our Gel Protein Recovery system.
Features and Specifications
  • The AALS II detergent is fully compatible with downstream applications (e.g. mass spectrometry).
  • The AALS II detergent allows solubilization of hydrophobic proteins and antibodies with the
    benefit of rapid and efficient detergent degradation.
  • Optimally buffered for gel protein recovery at pH 8.5.

Research Applications:
  • Allows improved recovery of hydrophobic proteins and antibodies from 1D and 2D
    polyacrylamide gels by electroelution.
  • Protein identification and characterization by mass spectrometry from gel based samples.
  • Top-down analysis of intact hydrophobic proteins and antibodies.

  • Colorless liquid solution
  • This product is for R and D use only; it is not for drug, household, or other uses.

Data Sets

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***Please to download documentation.

GPR Electroelution Buffer IV, Tris-Glycine Plus Availability
This product is discontinued and no longer for sale.

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GPR Electroelution Buffer IV, Tris-Glycine Plus

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