Ultrapure DAN MALDI Matrix

overview | features and specs | data sets | literature

Product Overview
Ultrapure 1,5 diaminonapthalene (DAN) is an important matrix used in peptide and protein analysis by matrix assisted laser desorption ionization (MALDI) mass spectrometry. The matrix is effective in promoting reduction of disulfide bonds in the gas phase, which greatly facilitates analysis of proteins and peptides containing disulfide linkages.

Alternative names:
1, 5-Naphthalenediamine
Features and Specifications
  • Improves CID fragmentation of proteins with disulfide linkages
  • Enhances in-source fragmentation of peptides
  • Easy reconstitution directly in vial for ready-to-use stock

Research Applications:
  • MS and MS/MS analysis of peptides and proteins with disulfide linkages
  • Peptide analysis in ISD (In Source Decay) experiments
  • Analysis of gangliosides by MALDI-MS

  • Each Ultrapure MALDI matrix is provided as a recrystallized and highly purified powder.
  • This product is for R&D use only; it is not for drug, household, or other uses.

Data Sets
Reduction of insulin using 1, 5 DAN Matrix
Insulin is composed of two peptide chains referred to as the A chain (21 amino acids - MW 2339) and B chain (30 amino acids - MW 3399). Insulin chains A and B are linked together by two disulfide bonds, and an additional disulfide is formed within the A chain. When insulin is spotted with CHCA matrix, the insulin subunits are poorly detected (upper panel). However, when insulin is spotted with DAN matrix, the disulfide bonds of insulin are cleaved during ionization, which releases the free A and B chains (lower panel).

(click image(s) above to enlarge)
***Please to download documentation.

Ultrapure DAN MALDI Matrix Availability
Ultrapure DAN MALDI Matrix
5 x 10 mg
CMS-104-55$78.00add to cart
Ultrapure DAN MALDI Matrix
10 x 10 mg
CMS-104-1010$132.00add to cart

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Ultrapure DAN MALDI Matrix

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