Progenta™ Zwitterionic Acid Labile Surfactant I (ZALS I)

overview | features and specs | data sets | literature

Product Overview
Progenta Zwitterionic Acid Labile Surfactants (ZALS) are novel, acid cleavable detergents that are fully compatible with mass spectrometry analysis. The Progenta ZALS's have been engineered to provide a safe alternative to detergents (e.g. CHAPS) that are commonly used in proteomics work, but that negatively impact subsequent analysis by mass spectrometry. While CHAPS and other detergents can improve protein solubility, they can be very difficult to remove during sample prep and purification of the protein sample. These bound detergents can cause significant impairment of protein analysis by mass spectrometry, as the surfactants can suppress analyte ion signal, promote analyte adduct formation, and present as contaminants during the analysis.

The Progenta Zwitterionic Acid Labile Surfactants are fully mass spec compatible and alleviate the problems commonly associated with CHAPS and other detergents in proteomics studies. At neutral pH, the Progenta ZALS functions as a powerful detergent for use in sample preparation, protein solubilization, and cell lysis protocols. After completing the experimental work, the solution is adjusted to a pH of 2.5 with trifluoroacetic acid (TFA) and incubated for 30 minutes to fully cleave the ZALS into small organic molecules that do not exhibit surfactant activity or interfere with analysis by mass spectrometry. Other commercially available acid labile surfactants require incubation in harsher acidic environments of pH 1.0 to 2.0 for up to several hours and may produce an oily pellet or film from the cleavage by-products. Progenta ZALS has been specially engineered to provide powerful surfactant performance with the added benefit of quick, simple acid-based detergent cleavage and removal for reproducible results in mass spectrometry-based proteomics studies.

Both Progenta Zwitterionic Acid Labile Surfactants are effective at solubilizing peptides and proteins, functioning in cell lysis protocols, optimizing enzymatic digestions, reducing surface adsorption losses via non-specific interactions, and replacing CHAPS in the isoelectric focusing (IEF) step of 2D gel separations. ZALS I has a Critical Micelle Concentration (CMC) of 3.4 mM. ZALS II has a shorter hydrophobic tail, yielding a less powerful CMC of 31.3 mM. Both ZALS I and II exhibit fast, efficient degradation kinetics at acidic pH's of 2.0 to 2.5.
Features and Specifications
Features:
  • Rapidly cleaved in just 30 minutes at pH 2.0 - 2.5 in 1% TFA
  • ZALS cleavage products are fully soluble and can be washed away to not interfere with mass spectrometry analysis
  • Promotes protein solubility and improves enzymatic digestion
  • Certified Mass Spec Grade


Research Applications:
  • Solubilization of proteins and other biomolecules
  • Sample preparation and solid phase extraction
  • Improved enzymatic digestion of proteins
  • Cell lysis and protein extraction from cell lines, tissues, and biological samples
  • 2D gel electrophoresis buffers
  • Extraction of biomolecules from environmental samples


Specifications:
  • Critical Micellular Concentration (CMC)

    ZALS I: CMC=3.4mM
  • Recommended concentrations for usage: 0.01 - 0.1%

Data Sets

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Literature
***Please to download documentation.

Progenta™ Zwitterionic Acid Labile Surfactant I (ZALS I) Availability
Progenta™ Zwitterionic Acid Labile Surfactant I (ZALS I)
5 mg
***this product must be shipped cold, requiring 1 or 2 day shipping
ALS-200-11$199.97add to cart
Progenta™ Zwitterionic Acid Labile Surfactant I (ZALS I)
5 x 5 mg
***this product must be shipped cold, requiring 1 or 2 day shipping
ALS-200-55$899.87add to cart
Progenta™ Zwitterionic Acid Labile Surfactant I (ZALS I)
10 x 5 mg
***this product must be shipped cold, requiring 1 or 2 day shipping
ALS-200-1010$1599.76add to cart


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Progenta™ Zwitterionic Acid Labile Surfactant I (ZALS I)

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