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Top-Down Proteomics Using High Resolution Mass Spectrometry and Online Core-Shell HPLC Protein Separation

Presented at the ASMS 2013 Sanibel Conference, this poster highlights top-down proteomics applications using a new, revolutionary core-shell- based HPLC column (Protea Amplus®) for online separation of proteins. This new technology combines the benefits of core shell silica particle design with large porous size providing the ideal solution for intact proteins analysis. With ultra low Eddy diffusion level due to the perfect packing and better coefficient of mass transfer, Amplus technology provides a flat van Deemter curve allowing a large application flexibility. The porosity of 300 Å allows proteins to have a very homogeneous, predictable and efficient interaction with the stationary phase.

The end result is enhanced separation of complex protein mixtures with limited peak dispersion of separated proteins.

...innovations for bioanalytics