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Quantifying Proteins in SDS-PAGE Using SuperBlue™ Coomassie Stains

A variety of techniques are available for the quantitation of proteins. Densitometry is particularly useful due to its sensitivity, accuracy, and versatility. In addition, the technique is cost-effective, simple, and readily available. Protea’s SuperBlue Coomassie stains are an excellent choice for visualizing proteins separated by SDS-PAGE gel electrophoresis. SuperBlue Coomassie Stains R250 and G250 are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis.

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