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LC-MALDI-TOF Analysis of Intact Proteins Electroeluted with the GPR-850

SDS-PAGE is an efficient method for separating intact proteins from complex biological samples. However, recovery of intact proteins for downstream mass spectrometry applications is often difficult, time consuming and typically only facilitates bottom-up approaches. Protea’s GPR-850 Gel Protein Recovery System offers an automated, multiplexed, microfluidics chip-based solution for the extraction of intact proteins from polyacrylamide gels.

In this application note, intact proteins from an E. coli lysate have been electroeluted from a 2D SDS-PAGE separation and analyzed via LC-MALDI-TOF.

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