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Improving Tryptic Digestions with Progenta Acid Labile Surfactants

Protea's Progenta Acid Labile Surfactants are novel, acid cleavable surfactants that facilitate quick, clean sample analysis free from the disruptive background noise commonly caused by molecules like SDS and CHAPS. Progenta surfactants are fully mass spec compatible and are engineered to provide a safe alternative to other surfactants commonly used in proteomic workflows. Major workflows into which these surfactants may be incorporated include sample solubilization and preparation, solid phase extractions, cell lysis and protein extraction, and enzymatic digestions. This document focuses on the benefits of integrating Progenta Acid Labile Surfactants into a standard enzymatic digestion protocol.

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