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Choosing PAGE Gel Concentration for Resolving the Protein of interest

Polyacrylamide gels can be formed with varying pore sizes for a wide range of protein separation. The size of the pores is dependent on the amount of total acrylamide per unit volume and the percentage of cross-linker available. The smaller pore size (higher percent acrylamide gel, e.g. 15%) is beneficial for separation of low molecular weight proteins and the larger pore size (lower percent acrylamide gel, e.g. 5%) is beneficial for separation of high molecular weight proteins.

The use of protein migration charts will aid in the selection of gel type that offers the optimum resolution of the protein of interest.


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