Posters and Publications

Mass Spectrometry Imaging Determines Biomarkers of Early Adaptive Precision Drug Resistance in Lung Cancer

Drug resistance emergence is a common problem that limits long term outcome benefits in the era of precision cancer therapy. Recently, we identified an early precision drug escape mechanism with adaptive tumor cellular reprogramming emerging within days after drug initiation (Fan W et al., 2011). Here we present a mass spectrometry imaging (MSI) approach to interrogate the biomolecular changes occurring within residual tumor cells under precision treatment with ALK-specific kinase inhibitor treatments in EML4-ALK fusion (ALK+) lung adenocarcinoma xenograft as well as in an ALK+ patient biopsy-derived cell line.

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Molecular Imaging of Biological Samples on Nanophotonic Laser Desorption Ionization Platforms High Throughput Screening of Urine Marker Codes by Laser Ablation Electrospray Ionization Mass Spectrometry (LAESI MS)
Cross Platform Lipid Imaging of Silver Nanoparticle-Exposed Rat Brain by LAESI-MS and MALDI-MS In situ Detection and Imaging of Ergot Alkaloids in Ipomea tricolor by LAESI-MS-MS and MALDI-MS
In situ Top Down Proteomics: Targeted Imaging of Proteins in Tissue Using Laser Ablation Electrospray Ionization Mass Spectrometry (LAESI-MS) Analysis of mixed biofilm (Staphylococcus aureus and Pseudomonas aeruginosa) by laser ablation electrospray ionization mass spectrometry
Observation of Subcellular Metabolite Gradients in Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry High-throughput quantitative analysis of domoic acid directly from mussel tissue using Laser Ablation Electrospray Ionization - tandem mass spectrometry
Combining Laser Ablation Electrospray Ionization Mass Spectrometry with Collision Cross Section for Improved Metabolite and Lipid Identification from Clinical Samples Direct Analysis of Animal and Plant Tissues by LAESI-MSI with Ion Mobility for Mapping of Metabolites and Lipids
Digging Deeper - Using Acid Labile Detergents to Uncover More of the Mass Spectrometry Imaging Proteome High Throughput Spatially Resolved Peptide Identification using Hydrogel Digestion and Electro-Elution
Increased Sensitivity in LAESI-MS Analyses using a Heated Extension Tube for the Direct Analysis of Plants Rapid Profiling of Arbuscular Mycorrhizal Fungi Spores Using LAESI-MS
Single Bead Peptide Microarrays Characterized by LAESI-MS MSI of Skin Wound Healing Biomarkers Using LAESI-MS
Toward single-cell analysis by plume collimation in laser ablation electrospray ionization mass spectrometry Relative quantitation in single-cell metabolomics by laser ablation electrospray mass spectrometry
Simultaneous detection of nonpolar and polar compounds by heat-assisted laser ablation electrospray ionization mass spectrometry Comparative local analysis of metabolites, lipids and proteins in intact fish tissues by LAESI mass spectrometry
Direct Sample Analysis Without Extraction Using LAESI-MS 2D and 3D Direct Analysis of Plant Tissues
Mass Spectrometry Imaging of Raclopride and Fexofenadine in Whole Body Mouse Tissue Using LAESI-MS A Lipidomic Imaging Analysis of Mouse Brain Using LAESI-MS in Positive and Negative Ion Modes Coupled with Ion Mobility Spectrometry
LAESI Mass Spectrometry Imaging of Contact Lens Spoilage Protein Profiling of Animal Tissues Using LAESI-MS
Three-Dimensional Imaging of Metabolites in Tissues under Ambient Conditions by Laser Ablation Electrospray Ionization Mass Spectrometry Direct Analysis of Lipids and Small Metabolites in Mouse Brain Tissue by AP IR-MALDI and Reactive LAESI-MS
Molecular Mass Spectrometry Imaging in Biomedical and Life Science Research Ambient mass spectrometry for in vivo local analysis and in situ molecular tissue imaging
Rapid, non-targeted discovery of biochemical transformation and biomarker candidates in oncovirus-infected cell lines using LAESI mass spectrometry Direct Analysis of Phycobilisomal Antenna Proteins and Metabolites in Small Cyanobacterial Populations by Laser Ablation Electrospray Ionization Mass Spectrometry
Internal energy deposition and ion fragmentation in atmospheric-pressure mid-infrared laser ablation electrospray ionization 2D and 3D Imaging of Biological Samples Using a Novel Integrated LAESI-MS Source
Overview: Laser Ablation Electrospray Ionization (LAESI) Metabolic Profiling of Biofluids by LAESI-MS
Direct 2D and 3D Metabolic Profiling of Flower Petals Using LAESI-MS Profiling metabolites and peptides in single cells
In Situ Cell-by-Cell Imaging and Analysis of Small Cell Populations by Mass Spectrometry Direct Detection of Diverse Metabolic Changes in Virally Transformed and Tax-Expressing Cells by Mass Spectrometry
Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
Direct analysis of lipids and small metabolites in mouse brain tissue by AP IR-MALDI and reactive LAESI mass spectrometry Minimally invasive monitoring of cellulose degradation by desorption electrospray ionization and laser ablation electrospray ionization mass spectrometry
Molecular mass spectrometry imaging in biomedical and life science research Simultaneous Imaging of Small Metabolites and Lipids in Rat Brain Tissues at Atmospheric Pressure by Laser Ablation Electrospray Ionization Mass Spectrometry
Laser Ablation Electrospray Ionization for Atmospheric Pressure Molecular Imaging Mass Spectrometry Ablation and analysis of small cell populations and single cells by consecutive laser pulses
In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry Three-Dimensional Imaging of Metabolites in Tissues under Ambient Conditions by Laser Ablation Electrospray Ionization Mass Spectrometry
In vitro analysis of metabolites from the untreated tissue of Torpedo californica electric organ by mid-infrared laser ablation electrospray ionization mass spectrometry Ambient Molecular Imaging and Depth Profiling of Live Tissue by Infrared Laser Ablation Electrospray Ionization Mass Spectrometry
Molecular imaging by Mid-IR laser ablation mass spectrometry Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry
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