Quantitative Mass Spectrometry Imaging of Rabbit Skin Topically Dosed with an Antifungal Compound using a Stable Isotope Labeled Internal Standard
Mass spectrometry imaging (MSI) allows for the determination of the localization of endogenous and exogenous compounds along with their relative concentrations in thin tissue sections. A major area of interest in the pharmaceutical industry is the determination of exactly how much of a dosed compound is getting into the target location for the compound to act. A considerable challenge of MSI of small molecules in dosing studies is in determining the absolute concentration of a compound at a given location in a tissue section. Here we present a strategy for absolute quantitation from the topical dosing of an antifungal compound in rabbit skin using a stable isotope labeled internal standard.
[presented at ASMS 2015]