Cross Platform Lipid Imaging of Silver Nanoparticle-Exposed Rat Brain by LAESI-MA and MALDI-MS
Silver nanoparticles (AgNP) are widely used in industrial, household, diagnostic and antimicrobial products. Emerging studies suggest that AgNP can biodistribute to the brain following inhalation and potentially be neurotoxic. However, the molecular effects of AgNP exposure have not been fully explored.
Here, we report the utility of differing MSI platforms in identifying rat brain lipidome changes following AgNP inhalation. The use of multiple mass spectrometry imaging (MSI) platforms has the advantage of both increasing and cross-validating the information gathered from individual MSI experiments. Lipid data are generated by classical matrix-assisted laser desorption/ionization (MALDI) and laser ablation electrospray ionization (LAESI) MSI platforms and illustrate complementary signatures between platforms. These data increase our understanding of possible health effects due to AgNP in common products.
[presented at ASMS 2015]